Callus induction and plant regeneration of tomato through anther culture

Abstract

Anthers of 15 genotypes were cultured on MS medium
having different concentration of auxins and cytokinins to
induce callusing. Of the different media compositions used,
callus induction was observed in eight genotypes on MS
medium supplemented with 2ip (1mg/l) and IAA (2mg/l).
The percent callus formation was maximum in genotype Wild
Texas (88.00) and minimum in TLBNRH-1214(4%). The calli
obtained from the anthers of the eight genotypes were
cultured on different regeneration media. However, the
regeneration was observed only in calli of Wild Texas with
percent regeneration of 0.66 on MS media supplemented
with zeatin (0.5mg/l). After rooting on MS medium containing
IBA (0.1mg/l), plantlets were hardened and transferred to
pots.