Utilizing Ogura-CMS and doubled haploid lines of cabbage for new-generation hybrid breeding
Keywords:Cabbage, CMS, doubled haploid, F1 hybrid, SSR.
In this study, we report the development of a uniform hybrid of cabbage by utilizing cytoplasmic male sterile (CMS) and doubled haploid (DH) genotypes. Firstly, 26 cabbage genotypes were subjected to molecular diversity analysis using 52 SSR primers. Most divergent parents representing CMS (6) and DH (5) genotypes were crossed in Line × Tester mating design during the summer of 2018. During winter, 2018-19, all the parents, their 30 F1’s, and one standard check cultivar (Pusa Hybrid-81) were evaluated for different horticultural traits. Based on the mean performance, three lines viz. KTCB-836A, KTCB-5A, KTCB-6A and two testers viz., KTCB-50-1 and KTCB-51-19 were found superior. Hence, these genotypes can be utilized in future cabbage hybrid breeding programs. While among the 30 DH-based hybrids, six cross combinations viz. KTCB-1A × KTCB-50-1 (586.82 q), KTCB-836A × KTCB-51-19 (568.93 q), KTCB-836A × KTCB-51-2 (557.27 q), KTCB-836A × KTCB-50-3 (527.90 q), KTCB-836A × KTCB-51-6 (517.08 q) and KTCB-836A × KTCB-50-1 (513.06 q) were found superior than the Standard check cultivar, Pusa Hybrid-81 (405.93 q). As these hybrids were quite uniform hence, after multilocations testing they can be released for commercial cultivation in India.