Callus induction and plant regeneration of tomato through anther culture

Authors

  • Sandeep Kumar Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004 Author
  • SK Jindal Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004 Author
  • NK Sarao School of Agricultural Biotechnology, Punjab Agricultural University, Ludhiana 141 004 Author
  • MS Dhaliwal Department of Vegetable Science, Punjab Agricultural University, Ludhiana 141 004 Author

DOI:

https://doi.org/10.61180/vegsci.2020.v47.i1.04

Keywords:

Growth regulators, organogenesis, Solanum lycopersicum L.

Abstract

Anthers of 15 genotypes were cultured on MS medium
having different concentration of auxins and cytokinins to
induce callusing. Of the different media compositions used,
callus induction was observed in eight genotypes on MS
medium supplemented with 2ip (1mg/l) and IAA (2mg/l).
The percent callus formation was maximum in genotype Wild
Texas (88.00) and minimum in TLBNRH-1214(4%). The calli
obtained from the anthers of the eight genotypes were
cultured on different regeneration media. However, the
regeneration was observed only in calli of Wild Texas with
percent regeneration of 0.66 on MS media supplemented
with zeatin (0.5mg/l). After rooting on MS medium containing
IBA (0.1mg/l), plantlets were hardened and transferred to
pots.

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Published

2020-06-30

Issue

Vol. 47 No. 01 (2020): Vegetable Science

Section

Articles

License

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

How to Cite

Kumar, S., Jindal, S., Sarao, N., & Dhaliwal, M. (2020). Callus induction and plant regeneration of tomato through anther culture. Vegetable Science, 47(01), 23-27. https://doi.org/10.61180/vegsci.2020.v47.i1.04

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