Effect of incubation temperature and sucrose concentration on callus induction and plant regeneration in cauliflower (Brassica oleracea var. botrytis)

Abstract

Androgenic and plant regeneration potential from anther
culture were investigated in cauliflower genotypes under
different incubation temperatures, sucrose concentrations
and regeneration media compositions. Anthers were
cultured on MS medium supplemented with 1.5 mg/L 2,4-D
and 1.0 mg/L NAA growth hormone with four different
treatments of sucrose i.e. 3%, 5%, 10% and 13%. Maximum
androgenesis has been observed with 10% sucrose
concentration. Anthers of all the varieties were incubated
under three different temperatures i.e. 25°C, 32°C and 35°C.
Among these incubation temperatures, higher androgenesis
was observed at 32°C incubation temperature. Callus were
cultured on five shoot regeneration media compositions,
among which maximum regeneration has been observed on
MS media supplemented with 5.0 mg/L BAP and 1.0 mg/L
NAA followed by MS media supplemented with 6.0 mg/L
BAP and 1.0 mg/L NAA. Maximum root regeneration has
been observed in ½ MS medium supplemented with 1.0 mg/
L IBA growth hormone followed by ½ MS medium
supplemented with 0.4 mg/L IBA. In cytological examination,
20.3% of the regenerated plants were found to be haploid
while 29.8% were spontaneous doubled haploids.