Micro and mini-tubers formation and in vitro shoot regeneration from bud sprout of potato (Solanum tuberosum L.)

Abstract

The present study was carried out to develop micropropagation protocol of potato using sprout as an explants. Explants were cultured on in Murashige and Skoog’s (MS; 1962) media supplemented with eleven different combination of Indole-3-butyric acid (IBA), Kinetin, 1-Naphthaleneacetic acid (NAA) and 2,4- Dichlorophenoxyacetic acid (2,4-D) for initiation of cultures. The treatment involving a combination of IBA, Kinetin, NAA and 2,4-D gave good response for growth of shoot. The regenerated shoots were sub-cultured further using nodal cutting as explants in same media for further multiplication. The developed plantlets were acclimatized in a green house. Acclimatized plants were transplanted in the soil for further growth and development which yielded 3-17 healthy mini-tubers . For microtuber production, high level of sucrose (8%) found promising results than low level of sucrose (3%).