Electrophoretic protein profile and ISSR marker based genetic relationship study in ridge gourd [Luffa acatangula (Roxb.)L.]

Abstract

An experiment was conducted to study the biochemical as
well as molecular traits among eight ridge gourd inbred lines
by using SDS-PAGE analysis and ISSR marker data. The
entire seed protein profile comprised of 24 protein bands,
distributed into four major zones A, B, C and D in the
increasing order of electrophoretic mobility as analysed by
SDS-PAGE. Maximum sixteen bands were found in PRG 120
and minimum thirteen bands were observed in PCPGR 7256
and PRG 142. Similarity index value ranged from 25% to
100%. Hundred percent similarities were observed for
genotype PCPGR 7256 with PRG 117. Genotype PRG 131
showed least similarity (25%) with PRG 132 as PRG 137 with
PRG 7. The UPGMA clustering divided all the genotypes
into two major clusters (I and II) with 43% similarity among
them. Cluster I comprised of 5 genotypes and cluster II
comprised of 3 genotypes. Among 20 ISSR markers, only 4
were selected due to their polymorphic bands. A total of 27
bands were produced and the number of loci ranged from 6
to 7. The per cent polymorphism varied from 66.67 to 85.71
with an average of 74.07. The similarity matrix value ranged
from 43% to 90%. The maximum similarity value was recorded
for PCPGR 7256 (0.90) with PRG 142. The lowest similarity
was recorded for PCPGR 7256 (0.43) with PRG 137. In ISSR
profile based dendogram eight genotypes were classified
into two main clusters. Cluster I comprised of 5 genotypes
with 62% similarity among themselves whereas cluster II
comprised of 3 genotypes with 67% similarity. The
information generated in the present study could be further
utilized for ridge gourd improvement programme in future.