Assessment of genetic purity of F1 hybrids and their parents through SDS-PASE in brinjal (Solanum melongena L.)

Abstract

Eight brinjal cultivars/genotypes and their 28 F1 hybrids were used in the current experiment to determine the genetic purity using SDS-PASE seed protein profiles. When 36 genotypes, including eight parents and 28 F1 hybrids, were examined using the sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), there was a significant variance in the number of protein bands. SDS-PAGE was used to determine which parents (male or female) the F1s hybrids were more closely linked to as well as the degree of purity between the parents and their hybrids. While Pusa Purple Long, BR-112 and CHFB-7 were weak female parents, Swarna Pratibha, NDB-3, Pant Rituraj and CHFB-6 were powerful female parents. Therefore, it would be much imperative to use parents viz., Swarna Pratibha, NDB-3, Pant Rituraj and CHFB-6 for crossing programmed to transfer desirable traits from parents to offspring through heterosis breeding procedures in brinjal.